frozen osteolytic murine sarcoma cells Search Results


osteolytic murine sarcoma cells  (ATCC)
94
ATCC osteolytic murine sarcoma cells
Osteolytic Murine Sarcoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine diversity  (Muris Inc)
86
Muris Inc murine diversity
Murine Diversity, supplied by Muris Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine single cell transcriptomic data  (Muris Inc)
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Muris Inc murine single cell transcriptomic data
Murine Single Cell Transcriptomic Data, supplied by Muris Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tabula muris compendium23  (Muris Inc)
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Muris Inc tabula muris compendium23
Tabula Muris Compendium23, supplied by Muris Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m143 protein of murine cytomegalovirus  (Biochemie GmbH)
90
Biochemie GmbH m143 protein of murine cytomegalovirus
M143 Protein Of Murine Cytomegalovirus, supplied by Biochemie GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tabula murix scrna seq data  (Muris Inc)
86
Muris Inc tabula murix scrna seq data
Tabula Murix Scrna Seq Data, supplied by Muris Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scrna-seq calico murine cell atlas  (Calico Labs)
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Calico Labs scrna-seq calico murine cell atlas
scAgeCom was built by applying scDiffCom to 58 public <t>scRNA-seq</t> aging datasets encompassing 23 tissues ( y axis) from both male and female mice and different experimental techniques ( x axis). The number of cell types detected per dataset is indicated in each rectangle. Information regarding the ages of the mice is indicated along the x axis and applies to all datasets of a given column unless specified otherwise in specific rectangles. Empty areas indicate unavailable data. Data are available in Supplementary Table . m, months.
Scrna Seq Calico Murine Cell Atlas, supplied by Calico Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine vls model  (Biomodels LLC)
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Biomodels LLC murine vls model
scAgeCom was built by applying scDiffCom to 58 public <t>scRNA-seq</t> aging datasets encompassing 23 tissues ( y axis) from both male and female mice and different experimental techniques ( x axis). The number of cell types detected per dataset is indicated in each rectangle. Information regarding the ages of the mice is indicated along the x axis and applies to all datasets of a given column unless specified otherwise in specific rectangles. Empty areas indicate unavailable data. Data are available in Supplementary Table . m, months.
Murine Vls Model, supplied by Biomodels LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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frcs  (ScienCell)
90
ScienCell frcs
scAgeCom was built by applying scDiffCom to 58 public <t>scRNA-seq</t> aging datasets encompassing 23 tissues ( y axis) from both male and female mice and different experimental techniques ( x axis). The number of cell types detected per dataset is indicated in each rectangle. Information regarding the ages of the mice is indicated along the x axis and applies to all datasets of a given column unless specified otherwise in specific rectangles. Empty areas indicate unavailable data. Data are available in Supplementary Table . m, months.
Frcs, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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murine hepatocytes primary murine hepatocytes  (Janvier Labs)
86
Janvier Labs murine hepatocytes primary murine hepatocytes
Figure 6: TXNIP upregulation upon depletion of PK activity in different model systems. (A) RT-qPCR quantification of Pklr (left) and Txnip (right) transcripts upon transient siRNA-mediated knockdown of Pklr in mouse primary <t>hepatocytes.</t> Each datapoint represents an average value of 4 technical replicates. Paired samples were derived from the same mouse and treated with non-targeting control or Pklr targeting siRNA; n ¼ 3, Paired samples Student’s t-test. (B) RT-qPCR quantification of PKLR (left), PKM (middle) and TXNIP (right) transcripts upon transient sequential siRNA-mediated knockdown of PKLR and/or PKM in HepaRG cells; n ¼ 4, pairwise Student’s t-test, p values adjusted with Bonferroni method, ns p 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. (C) RT-qPCR quantification of transcripts encoding pkm and txnip variants in wt and pkma/b double knocked out zebrafish embryos (day 5); n ¼ 3, Student’s t-test, ns p 0.05, *p < 0.05, **p < 0.01.
Murine Hepatocytes Primary Murine Hepatocytes, supplied by Janvier Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna murine naip5  (imaGenes GmbH)
90
imaGenes GmbH cdna murine naip5
Figure 6: TXNIP upregulation upon depletion of PK activity in different model systems. (A) RT-qPCR quantification of Pklr (left) and Txnip (right) transcripts upon transient siRNA-mediated knockdown of Pklr in mouse primary <t>hepatocytes.</t> Each datapoint represents an average value of 4 technical replicates. Paired samples were derived from the same mouse and treated with non-targeting control or Pklr targeting siRNA; n ¼ 3, Paired samples Student’s t-test. (B) RT-qPCR quantification of PKLR (left), PKM (middle) and TXNIP (right) transcripts upon transient sequential siRNA-mediated knockdown of PKLR and/or PKM in HepaRG cells; n ¼ 4, pairwise Student’s t-test, p values adjusted with Bonferroni method, ns p 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. (C) RT-qPCR quantification of transcripts encoding pkm and txnip variants in wt and pkma/b double knocked out zebrafish embryos (day 5); n ¼ 3, Student’s t-test, ns p 0.05, *p < 0.05, **p < 0.01.
Cdna Murine Naip5, supplied by imaGenes GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnase inhibitor, murine  (ApexBio)
90
ApexBio rnase inhibitor, murine
Figure 6: TXNIP upregulation upon depletion of PK activity in different model systems. (A) RT-qPCR quantification of Pklr (left) and Txnip (right) transcripts upon transient siRNA-mediated knockdown of Pklr in mouse primary <t>hepatocytes.</t> Each datapoint represents an average value of 4 technical replicates. Paired samples were derived from the same mouse and treated with non-targeting control or Pklr targeting siRNA; n ¼ 3, Paired samples Student’s t-test. (B) RT-qPCR quantification of PKLR (left), PKM (middle) and TXNIP (right) transcripts upon transient sequential siRNA-mediated knockdown of PKLR and/or PKM in HepaRG cells; n ¼ 4, pairwise Student’s t-test, p values adjusted with Bonferroni method, ns p 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. (C) RT-qPCR quantification of transcripts encoding pkm and txnip variants in wt and pkma/b double knocked out zebrafish embryos (day 5); n ¼ 3, Student’s t-test, ns p 0.05, *p < 0.05, **p < 0.01.
Rnase Inhibitor, Murine, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


scAgeCom was built by applying scDiffCom to 58 public scRNA-seq aging datasets encompassing 23 tissues ( y axis) from both male and female mice and different experimental techniques ( x axis). The number of cell types detected per dataset is indicated in each rectangle. Information regarding the ages of the mice is indicated along the x axis and applies to all datasets of a given column unless specified otherwise in specific rectangles. Empty areas indicate unavailable data. Data are available in Supplementary Table . m, months.

Journal: Nature Aging

Article Title: scDiffCom: a tool for differential analysis of cell–cell interactions provides a mouse atlas of aging changes in intercellular communication

doi: 10.1038/s43587-023-00514-x

Figure Lengend Snippet: scAgeCom was built by applying scDiffCom to 58 public scRNA-seq aging datasets encompassing 23 tissues ( y axis) from both male and female mice and different experimental techniques ( x axis). The number of cell types detected per dataset is indicated in each rectangle. Information regarding the ages of the mice is indicated along the x axis and applies to all datasets of a given column unless specified otherwise in specific rectangles. Empty areas indicate unavailable data. Data are available in Supplementary Table . m, months.

Article Snippet: are provided with this paper Datasets used in this study: • scRNA-seq Tabula Muris Senis: https://s3.console.aws.amazon.com/s3/buckets/czb-tabula-muris-senis • scRNA-seq Calico murine cell atlas: https://mca.research.calicolabs.com/ • LRIs from CellChat: available from their R package • LRIs from NicheNet: available from their R package • LRIs from SingleCellSignalR: available from their R package • LRIs from CellPhoneDB: https://www.cellphonedb.org/ • LRIs from CellTalkDB: https://github.com/ZJUFanLab/CellTalkDB • LRIs from connectomeDB2020: https://asrhou.github.io/NATMI/ • LRIs from ICELLNET: https://github.com/soumelis-lab/ICELLNET • General gene information: https://mygene.info/ and https://omnipathdb.org/ • GO terms: Ensembl (version 102), https://useast.ensembl.org/index.html • KEGG: accessed via the R package KEGGREST • gene2pubmed table: ftp://ftp.ncbi.nlm.nih.gov/gene/DATA/gene2pubmed.gz • GenAge (build 20): https://genomics.senescence.info/genes/index.html • LongevityMap (build 3): https://genomics.senescence.info/longevity/ • Microarray meta-analysis of Ageing Gene Expression database: https://genomics.senescence.info/gene_expression/ • CellAge (build 2): Supplementary Material of 10.1093/molbev/msab369 • Secretomics data for mBMM: Supplementary Material of 10.1126/science.1232578 • Secretomics data for mNeuron: Supplementary Material of 10.15252/embj.2020105693 • Secretomics data for mMSC-AT: Supplementary Material of 10.18632/aging.202423 • Secretomics data for rCM: Supplementary Material of 10.1161/circulationaha.119.044914 • Secretomics data for hUVEC: Supplementary Material of 10.1016/j.ajpath.2019.10.007 • Secretomics data for hPDE: Supplementary Material of 10.1002/pmic.202100320 Datasets created in this study: • scDiffCom LRIs: available from the R package scDiffCom ( https://github.com/CyrilLagger/scDiffCom/tree/master/data ) • Aging and sex scAgeCom results: 10.6084/m9.figshare.17074964 • Data to run the app scAgeComShiny and scagecom.org: https://figshare.com/articles/dataset/scAgeComShiny_data/17075375

Techniques:

Figure 6: TXNIP upregulation upon depletion of PK activity in different model systems. (A) RT-qPCR quantification of Pklr (left) and Txnip (right) transcripts upon transient siRNA-mediated knockdown of Pklr in mouse primary hepatocytes. Each datapoint represents an average value of 4 technical replicates. Paired samples were derived from the same mouse and treated with non-targeting control or Pklr targeting siRNA; n ¼ 3, Paired samples Student’s t-test. (B) RT-qPCR quantification of PKLR (left), PKM (middle) and TXNIP (right) transcripts upon transient sequential siRNA-mediated knockdown of PKLR and/or PKM in HepaRG cells; n ¼ 4, pairwise Student’s t-test, p values adjusted with Bonferroni method, ns p 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. (C) RT-qPCR quantification of transcripts encoding pkm and txnip variants in wt and pkma/b double knocked out zebrafish embryos (day 5); n ¼ 3, Student’s t-test, ns p 0.05, *p < 0.05, **p < 0.01.

Journal: Molecular metabolism

Article Title: Depletion of pyruvate kinase (PK) activity causes glycolytic intermediate imbalances and reveals a PK-TXNIP regulatory axis.

doi: 10.1016/j.molmet.2023.101748

Figure Lengend Snippet: Figure 6: TXNIP upregulation upon depletion of PK activity in different model systems. (A) RT-qPCR quantification of Pklr (left) and Txnip (right) transcripts upon transient siRNA-mediated knockdown of Pklr in mouse primary hepatocytes. Each datapoint represents an average value of 4 technical replicates. Paired samples were derived from the same mouse and treated with non-targeting control or Pklr targeting siRNA; n ¼ 3, Paired samples Student’s t-test. (B) RT-qPCR quantification of PKLR (left), PKM (middle) and TXNIP (right) transcripts upon transient sequential siRNA-mediated knockdown of PKLR and/or PKM in HepaRG cells; n ¼ 4, pairwise Student’s t-test, p values adjusted with Bonferroni method, ns p 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. (C) RT-qPCR quantification of transcripts encoding pkm and txnip variants in wt and pkma/b double knocked out zebrafish embryos (day 5); n ¼ 3, Student’s t-test, ns p 0.05, *p < 0.05, **p < 0.01.

Article Snippet: 2.1.3. siRNA transfection of murine hepatocytes Primary murine hepatocytes from 8 to 12-weeks-old C57BL6/N mice (Janvier Labs) were isolated using collagenase perfusion [48,49].

Techniques: Activity Assay, Quantitative RT-PCR, Knockdown, Derivative Assay, Control